Investigating effective factors of biocementation soil improvement on sandy soil with different Fine-content

Abstract
One of the most common methods of soil improvement is to use additives in order to improve strength properties and permeability of the soil. Cements or chemicals are usually used as binders for soil particles, which lead to increase the soil shear strength and reduce its hydraulic conductivity (i.e. permeability). Nevertheless, these materials are not suitable for soil improvement in the long term because they require significant natural resources. The use of cement and chemicals for soil improvement is expensive and time-consuming. Management of renewable natural resources (microorganisms and their products) could lead to solve geotechnical and environmental problems and achieve great economic benefits in the building industry. In addition, the application of microbial biotechnology in the building industry make easier some of the existing methods of construction. Using the latest microbial biotechnology, a new type of building materials, namely biocement, has been produced as an alternative to cement or chemicals. Biocementation is the improvement of strength and stiffness of rock and soil by using microbial activity and their products. The process of the formation of precipitates or biocement in the presence of microorganisms is called microbialy induced calcium precipitation (MICP). Biocement can be used in solid and liquid states. In the liquid state, biogrout can flow like water with very low viscosity. Therefore, compared to cement and chemicals, it will be transmitted into the soil, more easily. Naturally, biocement is formed in the presence of microorganisms in ambient temperature and thus, it requires less energy. Because of the abundance of microorganisms in the nature and easy to reproduce with low cost, this type of cement is sustainable. The Microorganisms that are suitable for the production of biocement are usually non-pathogenic and environment friendly. In addition, unlike cement, soil can be improved without disturbance of ground and the environment; since microorganisms can penetrate into the soil and grow in it. This dissertation aims to realize the effect of ground condition on the MICP process in non-cohesive soils. Since this method is still in the laboratory stage, for being used in practical projects, it is required to carry out laboratory experiments, including relative density and particle size distribution, to evaluate the performance of this method in different ground conditions. For this purpose, it was used from Sandy soil with different silt contents of 0%, 5%, 10%, 15% and 20% in two states of Loose (Dr = 40%) and dense (Dr = 100%) conditions in this research. The high urease activity and non-pathogenic bacteria S. Pasteurii was also used in the MICP process. In order to consider the soil conditions on the efficiency of this type of improvement method, uniaxial compressive test parameters and precipitated calcium carbonate content were investigated. According to the results, increasing of silt content from 0% to 20%, leads to reductions of 40% and 46% in precipitated calcium carbonate content, increases of 57% and 41% in the uniaxial strength and increases of 79% and 71% in the elasticity modulus of the samples in two loose and dense conditions, respectively. It seems that these changes were resulted from shrinking of the empty space and increasing of the contact area between the soil particles.

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1. Chu, J., Ivanov, V., He, J., Maeimi, M. & Wu, S. in Ground Improvement Case Histories: Chemical, Electrokinetic, Thermal and Bioengineering Methods 571–589 (2015).
2. Cheng, L. & Cord-Ruwisch, R. In situ soil cementation with ureolytic bacteria by surface percolation. Ecol. Eng. 42, 64–72 (2012).
3. Kim, D., Park, K. & Kim, D. Effects of ground conditions on microbial cementation in soils. Materials (Basel). 7, 143–156 (2013).
4. Cuthbert, M. O. et al. Controls on the rate of ureolysis and the morphology of carbonate precipitated by S. Pasteurii biofilms and limits due to bacterial encapsulation. Ecol. Eng. 41, 32–40 (2012).
5. Ng, W., Lee, M. & Hii, S. An overview of the factors affecting microbial-induced calcite precipitation and its potential application in soil improvement. World Acad. Sci. Eng. Technol. 62, 723–729 (2012).
6. Shahin, M. A., Cheng, L. & Mujah, D. Influence of Key Environmental Conditions on Microbially Induced Cementation for Soil Stabilization. J. Geotech. Geoenvironmental Eng. 4016083 (2016).
7. Park, S.-S., Choi, S.-G. & Nam, I.-H. Effect of plant-induced calcite precipitation on the strength of sand. J. Mater. Civ. Eng. 26, 6014017 (2014).
8. Feng, K. & Montoya, B. M. Drained Shear Strength of MICP Sand at Varying Cementation Levels. Ifcee 2242–2251 (2015).
9. Rowshanbakht, K., Khamehchiyan, M., Sajedi, R. H. & Nikudel, M. R. Soil Improvement using calcium carbonate precipitation and the Effective Factors on it. J. Iran. Assoc. Eng. Geol. 8, 1–12 (in persian)
10. Rowshanbakht, K., Khamehchiyan, M., Sajedi, R. H. & Nikudel, M. R. Effect of injected bacterial suspension volume and relative density on carbonate precipitation resulting from microbial treatment. Ecol. Eng. 89, 49–55 (2016).
11. Ivanov, V. & Stabnikov, V. in Construction Biotechnology 109–138 (Springer, 2017).
12. Ivanov, V. Environmental microbiology for engineers. (CRC Press, 2015).
13. Yasuhara, H., Hayashi, K. & Okamura, M. in Geo-Frontiers 2011: Advances in Geotechnical Engineering 3984–3992 (2011).
14. Carmona, J. P. S. F., Oliveira, P. J. V. & Lemos, L. J. L. Biostabilization of a Sandy Soil Using Enzymatic Calcium Carbonate Precipitation. Procedia Eng. 143, 1301–1308 (2016).
15. Zhao, Q. et al. Factors affecting improvement of engineering properties of micp-treated soil catalyzed by bacteria and urease. J. Mater. Civ. Eng. 26, 4014094 (2014).
16. PTCC. Persian Type Culture Collection. Available at:http://ptcc.irost.org/DBank-details.asp?id=27&code=0. (Accessed: 4th March 2017)
17. ATCC. Sporosarcina pasteurii (Miquel) Yoon et al. ATCC ® 11859TM. Available at: https://www.lgcstandards-atcc.org/products/all/11859.aspx?geo_country=de#culturemethod. (Accessed: 4th March 2017)
18. Li, S. A laboratory study of the effects of bio-stabilization on geomaterials. (2013).
19. Lin, H., Suleiman, M. T., Brown, D. G. & Kavazanjian, E. Mechanical Behavior of Sands Treated by Microbially Induced Carbonate Precipitation. J. Geotech. Geoenvironmental Eng. 142, 04015066 (2015).
20. Kalantary, F. & Kahani, M. Evaluation of the Ability to Control Biological Precipitation to Improve Sandy Soils. Procedia Earth Planet. Sci. 15, 278–284 (2015).
21. Ivanov, V. & Stabnikov, V. in Construction Biotechnology 1–22 (Springer, 2017).