Transplantation of Autologous Adipose Derived Mesenchymal Stem Cells for Improvement of Quality of Life in Osteoarthritis Patients

Shafaei, Hajar; Bagernezhad, Hassan; Soliemanlou, Hossein; Poureisa, Masoud

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دوره 1، شماره 3 - ( ) جلد 1 شماره 3 صفحات 109-116 | برگشت به فهرست نسخه ها

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Shafaei H, Bagernezhad H, Soliemanlou H, Poureisa M. Transplantation of Autologous Adipose Derived Mesenchymal Stem Cells for Improvement of Quality of Life in Osteoarthritis Patients. IJMPP. 1 (3) :109-116
URL: http://journals.modares.ac.ir/article-32-3014-fa.html

Shafaei Hajar، Bagernezhad Hassan، Soliemanlou Hossein، Poureisa Masoud. Transplantation of Autologous Adipose Derived Mesenchymal Stem Cells for Improvement of Quality of Life in Osteoarthritis Patients. . 1 (3) :109-116

URL: http://journals.modares.ac.ir/article-32-3014-fa.html

Transplantation of Autologous Adipose Derived Mesenchymal Stem Cells for Improvement of Quality of Life in Osteoarthritis Patients

Hajar Shafaei¹، Hassan Bagernezhad²، Hossein Soliemanlou³، Masoud Poureisa⁴

1- Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
2- Bonyan Cellule Kosar Company, East Azarbaijan Science and Technology Park, Tabriz, Iran.
3- Orthopedic ward, Shahid Mahallati Hospital, Tabriz, Iran.
4- Department of Radiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

چکیده: (1204 مشاهده)

Background: Osteoarthritis (OA) has been recognized, as the most common inflammatory disease in the world. Adipose Stem Cells (ASCs), as a new feasible source with high numbers of stem cells and proliferative capacity have been used for regenerative medicine. Based immunomodulatory and chondrogenic properties of ASCs, this study aimed to assess intra articular injection of ASC effect on improvement of osteoarthritis signs. Methods and Materials: Adipose tissue samples were obtained from subcutaneous of abdomen. ASCs were isolated and cultured for at least three passages in culture media containing autologous serum and expanded them to 15-20 × 10⁶ cell. The morphology and proliferative potency of ASCs were determined. Immuno phenotype characteristics of ASCs were analyzed by flow-cytometry. Then cell suspensions were injected into knee articular spaces. After 6 months the function of knee was assessed by WOMAC, KOOS, Lysholm and Lequesne indexes. Results: The results of this study showed that homogenous spindle-shape ASCs expanded rapidly with low doubling time. The low expression of CD14 and CD45 indicated that ASCs are non hematopoietic cells and expressed high percentages of CD44, CD105 and CD90. Our results showed that injected ASCs were effective in improvement of OA by scoring systems for evaluation of pain, joint movements and daily physical activities were significantly changed due to injection of stem cells. Osteoarthritis severity indexes means of WOMAC and Lequesne were decreased from 53 to 12.3 and 15.1 to 2.1 respectively. Also osteoarthritis improvement indexes Lysholm & KOOS means were significantly increased from 35 to 15.1 and 70 to 126.7 respectively. In six months follow up of intra articular injection of ASCs, we observed no local or systemic side effect. After ASCs injection, walking distance considerably increased. The flexion angle of knee improved by 20-30 degrees compares to before of treatment. Conclusion: Autologous ASCs injection could be resulted in increasing of knee function, alleviated of pain and quality of life improvement.

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دریافت: ۱۳۹۵/۵/۱۱ | پذیرش: ۱۳۹۵/۶/۱۶ | انتشار: ۱۳۹۵/۶/۲۵